SYBR Green qPCR primers for detecting human ROS1 (ROS proto-oncogene 1, receptor tyrosine kinase) gene expression by real-time PCR.
Human ROS1 qPCR Primer Set – ROS proto-oncogene 1, receptor tyrosine kinase
Also known as: MCF3, ROS, c-ros-1
About this gene: This proto-oncogene, highly-expressed in a variety of tumor cell lines, belongs to the sevenless subfamily of tyrosine kinase insulin receptor genes. The ROS1 protein is a type I integral membrane protein with tyrosine kinase activity. The protein may function as a growth or differentiation factor receptor.
Gene details: NCBI Gene ID: 6098; HGNC ID: HGNC:10261; UniProt: P08922; RefSeq (MANE Select): NM_001378902.1; Chromosome location: 6q22.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.