SYBR Green qPCR primers for detecting human PYGL (glycogen phosphorylase L) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human PYGL qPCR Primer Set – glycogen phosphorylase L
Also known as: GSD6
About this gene: PYGL encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. The PYGL protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls.
Gene details: NCBI Gene ID: 5836; HGNC ID: HGNC:9725; UniProt: P06737; RefSeq (MANE Select): NM_002863.5; Chromosome location: 14q22.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.