SYBR Green qPCR primers for detecting human PTCH1 (patched 1, also called PTC) gene expression by real-time PCR.
Human PTCH1 qPCR Primer Set – patched 1
Also known as: BCNS, BCNS1, NBCCS, PTC, PTC1, PTCH, SLC65B1
About this gene: PTCH1 belongs to the patched family of proteins and a component of the hedgehog signaling pathway. Hedgehog signaling is important in embryonic development and tumorigenesis. The PTCH1 protein is the receptor for the secreted hedgehog ligands, which include sonic hedgehog, indian hedgehog and desert hedgehog.
Gene details: NCBI Gene ID: 5727; HGNC ID: HGNC:9585; UniProt: Q13635; RefSeq (MANE Select): NM_000264.5; Chromosome location: 9q22.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.