SYBR Green qPCR primers for detecting human PRKACA (protein kinase cAMP-activated catalytic subunit alpha) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human PRKACA qPCR Primer Set – protein kinase cAMP-activated catalytic subunit alpha
Also known as: CAFD1, PKACA, PPNAD4
About this gene: PRKACA encodes one of the catalytic subunits of protein kinase A, which exists as a tetrameric holoenzyme with two regulatory subunits and two catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. cAMP-dependent phosphorylation of proteins by protein kinase A is important to many cellular processes, including differentiation, proliferation, and apoptosis. Constitutive activation of PRKACA caused either by somatic mutations, or genomic duplications of regions that include PRKACA, have been linked to hyperplasias and adenomas of the adrenal cortex and are linked to corticotropin-independent Cushing's syndrome.
Gene details: NCBI Gene ID: 5566; HGNC ID: HGNC:9380; UniProt: P17612; RefSeq (MANE Select): NM_002730.4; Chromosome location: 19p13.12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.