SYBR Green qPCR primers for detecting human POU5F1 (POU class 5 homeobox 1, also called OCT4) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human POU5F1 qPCR Primer Set – POU class 5 homeobox 1
Also known as: OCT3, OCT4, OCT4Borf1, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
About this gene: POU5F1 encodes a transcription factor containing a POU homeodomain that is central to embryonic development and stem cell pluripotency. Aberrant expression of POU5F1 in adult tissues is linked to tumorigenesis. POU5F1 can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation.
Gene details: NCBI Gene ID: 5460; HGNC ID: HGNC:9221; UniProt: Q01860; RefSeq (MANE Select): NM_002701.6; Chromosome location: 6p21.33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.