SYBR Green qPCR primers for detecting human POLR2I (RNA polymerase II subunit I, also called RPB14.5) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human POLR2I qPCR Primer Set – RNA polymerase II subunit I
Also known as: RPB9, hRPB14.5
About this gene: POLR2I encodes a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with two other polymerase subunits, forms the DNA binding domain of the polymerase.
Gene details: NCBI Gene ID: 5438; HGNC ID: HGNC:9196; UniProt: P36954; RefSeq (MANE Select): NM_006233.5; Chromosome location: 19q13.12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.