SYBR Green qPCR primers for detecting human POLI (DNA polymerase iota, also called Eta2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human POLI qPCR Primer Set – DNA polymerase iota
Also known as: RAD30B, RAD3OB, eta2
About this gene: The POLI protein is an error-prone DNA polymerase involved in DNA repair. The POLI protein promotes DNA synthesis across lesions in the template DNA, which other polymerases cannot do. The encoded polymerase inserts deoxynucleotides across lesions and then relies on DNA polymerase zeta to extend the nascent DNA strand to bypass the lesion.
Gene details: NCBI Gene ID: 11201; HGNC ID: HGNC:9182; UniProt: Q9UNA4; RefSeq (MANE Select): NM_007195.3; Chromosome location: 18q21.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.