SYBR Green qPCR primers for detecting human PDF (peptide deformylase, mitochondrial) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human PDF qPCR Primer Set – peptide deformylase, mitochondrial
About this gene: Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of PDF, peptide deformylase, removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase.
Gene details: NCBI Gene ID: 64146; HGNC ID: HGNC:30012; UniProt: Q9HBH1; RefSeq (MANE Select): NM_022341.2; Chromosome location: 16q22.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.