SYBR Green qPCR primers for detecting human ATP5F1E (ATP synthase F1 subunit epsilon) gene expression by real-time PCR.
Human ATP5F1E qPCR Primer Set – ATP synthase F1 subunit epsilon
Also known as: ATP5E, ATPE, MC5DN3
About this gene: ATP5F1E encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel.
Gene details: NCBI Gene ID: 514; HGNC ID: HGNC:838; UniProt: P56381; RefSeq (MANE Select): NM_006886.4; Chromosome location: 20q13.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.