SYBR Green qPCR primers for detecting human NT5M (5',3'-nucleotidase, mitochondrial) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NT5M qPCR Primer Set – 5',3'-nucleotidase, mitochondrial
Also known as: dNT-2, dNT2, mdN
About this gene: NT5M encodes a 5' nucleotidase that localizes to the mitochondrial matrix. This enzyme dephosphorylates the 5'- and 2'-phosphates of uracil and thymine deoxyribonucleotides. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Gene details: NCBI Gene ID: 56953; HGNC ID: HGNC:15769; UniProt: Q9NPB1; RefSeq (MANE Select): NM_020201.4; Chromosome location: 17p11.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.