SYBR Green qPCR primers for detecting human NT5C (5', 3'-nucleotidase, cytosolic) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NT5C qPCR Primer Set – 5', 3'-nucleotidase, cytosolic
Also known as: DNT, DNT1, HEL74, P5N2, PN-I, PN-II, UMPH2, cdN, dNT-1
About this gene: NT5C encodes a nucleotidase that catalyzes the dephosphorylation of the 5' deoxyribonucleotides and 2'-dNTP and ribonucleotides, but not 5' ribonucleotides. Of the different forms of nucleotidases characterized, this enzyme is unique in its preference for 5'-dNTP. It may be one of the enzymes involved in regulating the size of dNTP pools in cells.
Gene details: NCBI Gene ID: 30833; HGNC ID: HGNC:17144; UniProt: Q8TCD5; RefSeq (MANE Select): NM_014595.3; Chromosome location: 17q25.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.