SYBR Green qPCR primers for detecting human NISCH (nischarin, also called IR1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NISCH qPCR Primer Set – nischarin
Also known as: I-1, IR1, IRAS, hIRAS
About this gene: NISCH encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the inner layer of the plasma membrane. The orthologous mouse protein is reported to influence cytoskeletal organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein is reported to bind to the adapter insulin receptor substrate 4 to mediate translocation of alpha-5 integrin from the cell membrane to endosomes.
Gene details: NCBI Gene ID: 11188; HGNC ID: HGNC:18006; UniProt: Q9Y2I1; RefSeq (MANE Select): NM_007184.4; Chromosome location: 3p21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.