SYBR Green qPCR primers for detecting human NAGS (N-acetylglutamate synthase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NAGS qPCR Primer Set – N-acetylglutamate synthase
Also known as: AGAS, ARGA
About this gene: The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I, the first enzyme of the urea cycle in mammals. NAGS may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been linked to hyperammonemia.
Gene details: NCBI Gene ID: 162417; HGNC ID: HGNC:17996; UniProt: Q8N159; RefSeq (MANE Select): NM_153006.3; Chromosome location: 17q21.31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.