SYBR Green qPCR primers for detecting human NAGPA (N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NAGPA qPCR Primer Set – N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase
Also known as: APAA, UCE
About this gene: Hydrolases are transported to lysosomes after binding to mannose 6-phosphate receptors in the trans-Golgi network. NAGPA encodes a enzyme that catalyzes the second step in the formation of the mannose 6-phosphate recognition marker on lysosomal hydrolases. Commonly known as 'uncovering enzyme' or UCE, this enzyme removes N-acetyl-D-glucosamine residues from GlcNAc-alpha-P-mannose moieties and thereby produces the recognition marker.
Gene details: NCBI Gene ID: 51172; HGNC ID: HGNC:17378; UniProt: Q9UK23; RefSeq (MANE Select): NM_016256.4; Chromosome location: 16p13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.