SYBR Green qPCR primers for detecting human ASCL1 (achaete-scute family bHLH transcription factor 1, also called hASH1) gene expression by real-time PCR.
Human ASCL1 qPCR Primer Set – achaete-scute family bHLH transcription factor 1
Also known as: ASH1, HASH1, MASH1, bHLHa46
About this gene: ASCL1 belongs to the basic helix-loop-helix family of transcription factors. The protein activates transcription by binding to the E box. Dimerization with other BHLH proteins is required for efficient DNA binding.
Gene details: NCBI Gene ID: 429; HGNC ID: HGNC:738; UniProt: P50553; RefSeq (MANE Select): NM_004316.4; Chromosome location: 12q23.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.