SYBR Green qPCR primers for detecting human NUDT15 (nudix hydrolase 15, also called MTH2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human NUDT15 qPCR Primer Set – nudix hydrolase 15
Also known as: MTH2, NUDT15D
About this gene: NUDT15 encodes a enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity.
Gene details: NCBI Gene ID: 55270; HGNC ID: HGNC:23063; UniProt: Q9NV35; RefSeq (MANE Select): NM_018283.4; Chromosome location: 13q14.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.