SYBR Green qPCR primers for detecting human MRPL17 (mitochondrial ribosomal protein L17, also called L17mt) gene expression by real-time PCR.
Human MRPL17 qPCR Primer Set – mitochondrial ribosomal protein L17
Also known as: L17mt, LIP2, MRP-L17, MRP-L26, RPL17L, RPML26, bL17m
About this gene: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.
Gene details: NCBI Gene ID: 63875; HGNC ID: HGNC:14053; UniProt: Q9NRX2; RefSeq (MANE Select): NM_022061.4; Chromosome location: 11p15.4
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.