SYBR Green qPCR primers for detecting human MMP11 (matrix metallopeptidase 11, also called ST3) gene expression by real-time PCR.
Human MMP11 qPCR Primer Set – matrix metallopeptidase 11
Also known as: SL-3, ST3, STMY3
About this gene: Proteins of the matrix metalloproteinase family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by MMP11 is activated intracellularly by furin within the constitutive secretory pathway.
Gene details: NCBI Gene ID: 4320; HGNC ID: HGNC:7157; UniProt: P24347; RefSeq (MANE Select): NM_005940.5; Chromosome location: 22q11.23
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.