SYBR Green qPCR primers for detecting human MGAT1 (alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase, also called GNT-I) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human MGAT1 qPCR Primer Set – alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase
Also known as: GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, GnTI, MGAT
About this gene: There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein.
Gene details: NCBI Gene ID: 4245; HGNC ID: HGNC:7044; UniProt: P26572; RefSeq (MANE Select): NM_002406.4; Chromosome location: 5q35.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.