SYBR Green qPCR primers for detecting human MDFI (MyoD family inhibitor) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human MDFI qPCR Primer Set – MyoD family inhibitor
Also known as: I-MF, I-mfa
About this gene: The MDFI protein is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures.
Gene details: NCBI Gene ID: 4188; HGNC ID: HGNC:6967; UniProt: Q99750; RefSeq (MANE Select): NM_005586.4; Chromosome location: 6p21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.