SYBR Green qPCR primers for detecting human MCCC2 (methylcrotonyl-CoA carboxylase subunit 2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human MCCC2 qPCR Primer Set – methylcrotonyl-CoA carboxylase subunit 2
Also known as: MCCB, MCCCbeta
About this gene: MCCC2 encodes a small subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme acts as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in MCCC2 are linked to 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.
Gene details: NCBI Gene ID: 64087; HGNC ID: HGNC:6937; UniProt: Q9HCC0; RefSeq (MANE Select): NM_022132.5; Chromosome location: 5q13.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.