SYBR Green qPCR primers for detecting human AFG1L (AFG1 like ATPase) gene expression by real-time PCR.
Human AFG1L qPCR Primer Set – AFG1 like ATPase
Also known as: AFG1, LACE1, c222389
About this gene: AFG1L encodes a mitochondrial integral membrane protein that is involved in mitochondrial protein homeostasis. The protein contains a P-loop motif and a five-domain structure that is conserved in fly, yeast, and bacteria. The AFG1L gene product functions to mediate the degradation of nuclear-encoded complex IV subunits.
Gene details: NCBI Gene ID: 246269; HGNC ID: HGNC:16411; UniProt: Q8WV93; RefSeq (MANE Select): NM_145315.5; Chromosome location: 6q21
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.