SYBR Green qPCR primers for detecting human KRT5 (keratin 5, also called K5) gene expression by real-time PCR.
Human KRT5 qPCR Primer Set – keratin 5
Also known as: CK5, DDD, DDD1, EBS1, EBS2, EBS2A, EBS2B, EBS2C, EBS2D, EBS2E, EBS2F, K5
About this gene: The KRT5 protein belongs to the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14.
Gene details: NCBI Gene ID: 3852; HGNC ID: HGNC:6442; UniProt: P13647; RefSeq (MANE Select): NM_000424.4; Chromosome location: 12q13.13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.