SYBR Green qPCR primers for detecting human KRT2 (keratin 2, also called K2e) gene expression by real-time PCR.
Human KRT2 qPCR Primer Set – keratin 2
Also known as: CK-2e, K2e, KRT2A, KRT2E, KRTE
About this gene: The KRT2 protein belongs to the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is expressed largely in the upper spinous layer of epidermal keratinocytes and mutations in KRT2 have been linked to bullous congenital ichthyosiform erythroderma.
Gene details: NCBI Gene ID: 3849; HGNC ID: HGNC:6439; UniProt: P35908; RefSeq (MANE Select): NM_000423.3; Chromosome location: 12q13.13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.