SYBR Green qPCR primers for detecting human FAN1 (FANCD2 and FANCI associated nuclease 1, also called hFAN1) gene expression by real-time PCR.
Human FAN1 qPCR Primer Set – FANCD2 and FANCI associated nuclease 1
Also known as: KIAA1018, KMIN, MTMR15, hFAN1
About this gene: FAN1 is involved in DNA interstrand cross-link repair and encodes a protein with 5' flap endonuclease and 5'-3' exonuclease activity. Mutations in FAN1 cause karyomegalic interstitial nephritis.
Gene details: NCBI Gene ID: 22909; HGNC ID: HGNC:29170; UniProt: Q9Y2M0; RefSeq (MANE Select): NM_014967.5; Chromosome location: 15q13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.