SYBR Green qPCR primers for detecting human ITIH1 (inter-alpha-trypsin inhibitor heavy chain 1, also called SHAP) gene expression by real-time PCR.
Human ITIH1 qPCR Primer Set – inter-alpha-trypsin inhibitor heavy chain 1
Also known as: H1P, IATIH, ITI-HC1, ITIH, SHAP
About this gene: ITIH1 belongs to the inter-alpha-trypsin inhibitor family of proteins. The heavy chain also interacts with hyaluronan, and this interaction may play a role in ovulation and fertilization, and has been implicated in multiple inflammatory diseases. ITIH1 is present in a gene cluster on chromosome 3.
Gene details: NCBI Gene ID: 3697; HGNC ID: HGNC:6166; UniProt: P19827; RefSeq (MANE Select): NM_002215.4; Chromosome location: 3p21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.