SYBR Green qPCR primers for detecting human EIF5B (eukaryotic translation initiation factor 5B, also called eIF-5B) gene expression by real-time PCR.
Human EIF5B qPCR Primer Set – eukaryotic translation initiation factor 5B
Also known as: IF2
About this gene: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors.
Gene details: NCBI Gene ID: 9669; HGNC ID: HGNC:30793; UniProt: O60841; RefSeq (MANE Select): NM_015904.4; Chromosome location: 2q11.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.