SYBR Green qPCR primers for detecting human IER3 (immediate early response 3) gene expression by real-time PCR.
Human IER3 qPCR Primer Set – immediate early response 3
Also known as: DIF-2, DIF2, GLY96, IEX-1, IEX-1L, IEX1, PRG1
About this gene: IER3 functions in the protection of cells from Fas- or tumor necrosis factor type alpha-induced apoptosis. Partially degraded and unspliced transcripts are found after virus infection in vitro, but these transcripts are not found in vivo and do not generate a valid protein.
Gene details: NCBI Gene ID: 8870; HGNC ID: HGNC:5392; UniProt: P46695; RefSeq (MANE Select): NM_003897.4; Chromosome location: 6p21.33
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.