SYBR Green qPCR primers for detecting human CXXC4 (CXXC finger protein 4) gene expression by real-time PCR.
Human CXXC4 qPCR Primer Set – CXXC finger protein 4
Also known as: IDAX
About this gene: CXXC4 encodes a CXXC-type zinc finger domain-containing protein that acts as an antagonist of the canonical wingless/integrated signaling pathway. The CXXC4 protein negatively regulates wingless/integrated signaling through interaction with the post synaptic density protein/ Drosophila disc large tumor suppressor/ zonula occludens-1 protein domain of Dishevelled, a scaffolding protein required for the stabilization of the transcriptional co-activator beta-catenin. In addition, the CXXC domain of this protein is reported to bind unmethylated CpG dinucleotides, localize to promoters and CpG islands, and interact with the catalytic domain of methylcytosine dioxygenase ten-eleven-translocation 2.
Gene details: NCBI Gene ID: 80319; HGNC ID: HGNC:24593; RefSeq (MANE Select): NM_025212.4; Chromosome location: 4q24
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.