SYBR Green qPCR primers for detecting human IARS1 (isoleucyl-tRNA synthetase 1, also called IRS) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human IARS1 qPCR Primer Set – isoleucyl-tRNA synthetase 1
Also known as: GRIDHH, IARS, ILERS, ILRS, IRS, PRO0785
About this gene: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Isoleucine-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family and has been identified as a target of autoantibodies in the autoimmune disease polymyositis/dermatomyositis.
Gene details: NCBI Gene ID: 3376; HGNC ID: HGNC:5330; UniProt: P41252; RefSeq (MANE Select): NM_002161.6; Chromosome location: 9q22.31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.