SYBR Green qPCR primers for detecting human HUS1 (HUS1 checkpoint clamp component, also called hHUS1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human HUS1 qPCR Primer Set – HUS1 checkpoint clamp component
Also known as: hHUS1
About this gene: The HUS1 protein is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. The HUS1 protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C, which loads the combined complex onto the chromatin.
Gene details: NCBI Gene ID: 3364; HGNC ID: HGNC:5309; UniProt: O60921; RefSeq (MANE Select): NM_004507.4; Chromosome location: 7p12.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.