SYBR Green qPCR primers for detecting human HIKESHI (heat shock protein nuclear import factor hikeshi) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human HIKESHI qPCR Primer Set – heat shock protein nuclear import factor hikeshi
Also known as: C11orf73, HLD13, HSPC138, HSPC179, L7RN6, OPI10
About this gene: HIKESHI encodes a evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition.
Gene details: NCBI Gene ID: 51501; HGNC ID: HGNC:26938; UniProt: Q53FT3; RefSeq (MANE Select): NM_016401.4; Chromosome location: 11q14.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.