SYBR Green qPCR primers for detecting human SPOUT1 (SPOUT domain containing methyltransferase 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human SPOUT1 qPCR Primer Set – SPOUT domain containing methyltransferase 1
Also known as: C9orf114, CENP-32, CENP32, HSPC109, NEDGSB
About this gene: SPOUT1 enables S-adenosyl-L-methionine binding activity; miRNA binding activity; and rRNA-methyltransferase activity. It is involved in maintenance of centrosome location; miRNA processing; and rRNA base methylation. Acts upstream of with a positive effect on post-transcriptional regulation of gene expression.
Gene details: NCBI Gene ID: 51490; HGNC ID: HGNC:26933; UniProt: Q5T280; RefSeq (MANE Select): NM_016390.4; Chromosome location: 9q34.11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.