SYBR Green qPCR primers for detecting human IFT25 (intraflagellar transport 25, also called PP25) gene expression by real-time PCR.
Human IFT25 qPCR Primer Set – intraflagellar transport 25
Also known as: C1orf41, CFAP232, FAP232, HSPB11, HSPCO34, PP25
About this gene: IFT25 is predicted to enable metal ion binding activity. It is predicted to be involved in kidney development; smoothened signaling pathway; and spermatogenesis. It is predicted to act upstream of or within multiple processes, including left/right axis specification; lung development; and skeletal system development.
Gene details: NCBI Gene ID: 51668; HGNC ID: HGNC:25019; UniProt: Q9Y547; RefSeq (MANE Select): NM_016126.4; Chromosome location: 1p32.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.