SYBR Green qPCR primers for detecting human HSD3B1 (hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human HSD3B1 qPCR Primer Set – hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
Also known as: 3BETAHSD, HSD3B, HSDB3, HSDB3A, SDR11E1
About this gene: The HSD3B1 protein is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The HSD3B1 protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.
Gene details: NCBI Gene ID: 3283; HGNC ID: HGNC:5217; UniProt: P14060; RefSeq (MANE Select): NM_000862.3; Chromosome location: 1p12
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.