SYBR Green qPCR primers for detecting human IFFO1 (intermediate filament family orphan 1) gene expression by real-time PCR.
Human IFFO1 qPCR Primer Set – intermediate filament family orphan 1
Also known as: HOM-TES-103, IFFO
About this gene: IFFO1 belongs to the intermediate filament family. Intermediate filaments are proteins which are primordial components of the cytoskeleton and nuclear envelope. The proteins encoded by the members of IFFO1 family are evolutionarily and structurally related but have limited sequence homology, with the exception of the central rod domain.
Gene details: NCBI Gene ID: 25900; HGNC ID: HGNC:24970; UniProt: Q0D2I5; RefSeq (MANE Select): NM_001193457.2; Chromosome location: 12p13.31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.