SYBR Green qPCR primers for detecting human HLA-DQA1 (major histocompatibility complex, class II, DQ alpha 1, also called HLA-DCA) gene expression by real-time PCR.
Human HLA-DQA1 qPCR Primer Set – major histocompatibility complex, class II, DQ alpha 1
Also known as: CELIAC1, DQ-A1, DQA1, HLA-DQA, HLA-DQA1*
About this gene: HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. The HLA-DQA1 gene product plays a central role in the immune system by presenting peptides derived from extracellular proteins.
Gene details: NCBI Gene ID: 3117; HGNC ID: HGNC:4942; UniProt: P01909; RefSeq (MANE Select): NM_002122.5; Chromosome location: 6p21.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.