SYBR Green qPCR primers for detecting human H3-4 (H3.4 histone, cluster member, also called H3t) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human H3-4 qPCR Primer Set – H3.4 histone, cluster member
Also known as: H3.4, H3/g, H3C16, H3FT, H3t, HIST3H3
About this gene: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones. The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Gene details: NCBI Gene ID: 8290; HGNC ID: HGNC:4778; UniProt: Q16695; RefSeq (MANE Select): NM_003493.3; Chromosome location: 1q42.13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.