SYBR Green qPCR primers for detecting human HFE (homeostatic iron regulator, also called HLA-H) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human HFE qPCR Primer Set – homeostatic iron regulator
Also known as: HFE1, HH, HLA-H, MVCD7, TFQTL2
About this gene: The HFE protein is a membrane protein that is similar to MHC class I-type proteins and associates with beta2-microglobulin. The HFE gene product is thought that this protein functions to regulate iron absorption by regulating the interaction of the transferrin receptor with transferrin. The iron storage disorder, hereditary haemochromatosis, is a recessive genetic disorder that results from defects in HFE.
Gene details: NCBI Gene ID: 3077; HGNC ID: HGNC:4886; UniProt: Q30201; RefSeq (MANE Select): NM_000410.4; Chromosome location: 6p22.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.