SYBR Green qPCR primers for detecting human HEXA (hexosaminidase subunit alpha) gene expression by real-time PCR.
Human HEXA qPCR Primer Set – hexosaminidase subunit alpha
Also known as: TSD
About this gene: HEXA belongs to the glycosyl hydrolase 20 family of proteins. The encoded preproprotein is proteolytically processed to generate the alpha subunit of the lysosomal enzyme beta-hexosaminidase. This enzyme, together with the cofactor GM2 activator protein, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines.
Gene details: NCBI Gene ID: 3073; HGNC ID: HGNC:4878; UniProt: P06865; RefSeq (MANE Select): NM_000520.6; Chromosome location: 15q23
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.