SYBR Green qPCR primers for detecting human HCRT (hypocretin neuropeptide precursor, also called Hypocretin) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human HCRT qPCR Primer Set – hypocretin neuropeptide precursor
Also known as: NRCLP1, OX, PPOX
About this gene: HCRT encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis.
Gene details: NCBI Gene ID: 3060; HGNC ID: HGNC:4847; UniProt: O43612; RefSeq (MANE Select): NM_001524.1; Chromosome location: 17q21.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.