SYBR Green qPCR primers for detecting human H2AJ (H2A.J histone, also called H2a/j) gene expression by real-time PCR.
Human H2AJ qPCR Primer Set – H2A.J histone
Also known as: H2AFJ
About this gene: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones. The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
Gene details: NCBI Gene ID: 55766; HGNC ID: HGNC:14456; UniProt: Q9BTM1; RefSeq (MANE Select): NM_177925.5; Chromosome location: 12p12.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.