SYBR Green qPCR primers for detecting human GLI1 (GLI family zinc finger 1) gene expression by real-time PCR.
Human GLI1 qPCR Primer Set – GLI family zinc finger 1
Also known as: GLI, PAPA8, PPD1
About this gene: GLI1 belongs to the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop.
Gene details: NCBI Gene ID: 2735; HGNC ID: HGNC:4317; UniProt: P08151; RefSeq (MANE Select): NM_005269.3; Chromosome location: 12q13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.