SYBR Green qPCR primers for detecting human GEMIN8 (gem nuclear organelle associated protein 8) gene expression by real-time PCR.
Human GEMIN8 qPCR Primer Set – gem nuclear organelle associated protein 8
Also known as: FAM51A1
About this gene: The GEMIN8 protein is part of the SMN complex, which is necessary for spliceosomal snRNP assembly in the cytoplasm and pre-mRNA splicing in the nucleus. The GEMIN8 protein binds to both SMN1 and the GEMIN6/GEMIN7 heterodimer, mediating their interaction. The GEMIN8 protein is found in nuclear Gemini of Cajal bodies and in the cytoplasm.
Gene details: NCBI Gene ID: 54960; HGNC ID: HGNC:26044; UniProt: Q9NWZ8; RefSeq (MANE Select): NM_001042479.2; Chromosome location: Xp22.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.