SYBR Green qPCR primers for detecting human GDF11 (growth differentiation factor 11) gene expression by real-time PCR.
Human GDF11 qPCR Primer Set – growth differentiation factor 11
Also known as: BMP-11, BMP11, VHO
About this gene: GDF11 encodes a secreted ligand of the TGF-beta superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer.
Gene details: NCBI Gene ID: 10220; HGNC ID: HGNC:4216; UniProt: O95390; RefSeq (MANE Select): NM_005811.5; Chromosome location: 12q13.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.