SYBR Green qPCR primers for detecting human GCK (glucokinase, also called HK4) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human GCK qPCR Primer Set – glucokinase
Also known as: FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2, PNDM1
About this gene: GCK belongs to the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant.
Gene details: NCBI Gene ID: 2645; HGNC ID: HGNC:4195; UniProt: P35557; RefSeq (MANE Select): NM_000162.5; Chromosome location: 7p13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.