SYBR Green qPCR primers for detecting human GBF1 (golgi brefeldin A resistant guanine nucleotide exchange factor 1) gene expression by real-time PCR.
Human GBF1 qPCR Primer Set – golgi brefeldin A resistant guanine nucleotide exchange factor 1
Also known as: ARF1GEF, CMT2GG, CMTDI2, CMTDIA
About this gene: GBF1 belongs to the Sec7 domain family. The GBF1 protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The GBF1 protein is localized to the Golgi apparatus and is involved in vesicular trafficking by activating ADP ribosylation factor 1.
Gene details: NCBI Gene ID: 8729; HGNC ID: HGNC:4181; UniProt: Q92538; RefSeq (MANE Select): NM_001377137.1; Chromosome location: 10q24.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.