SYBR Green qPCR primers for detecting human GALE (UDP-galactose-4-epimerase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human GALE qPCR Primer Set – UDP-galactose-4-epimerase
Also known as: SDR1E1, THC13
About this gene: GALE encodes UDP-galactose-4-epimerase which catalyzes two distinct but analogous reactions: the epimerization of UDP-glucose to UDP-galactose, and the epimerization of UDP-N-acetylglucosamine to UDP-N-acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids. Mutations in GALE result in epimerase-deficiency galactosemia, also referred to as galactosemia type 3, a disease characterized by liver damage, early-onset cataracts, deafness and cognitive disability, with symptoms ranging from mild to severe.
Gene details: NCBI Gene ID: 2582; HGNC ID: HGNC:4116; UniProt: Q14376; RefSeq (MANE Select): NM_001008216.2; Chromosome location: 1p36.11
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.