SYBR Green qPCR primers for detecting human FLII (FLII actin remodeling protein) gene expression by real-time PCR.
Human FLII qPCR Primer Set – FLII actin remodeling protein
Also known as: CMD2J, FLI, FLIL, Fli1
About this gene: FLII encodes a protein with a gelsolin-like actin binding domain and an N-terminal leucine-rich repeat-protein protein interaction domain. The protein is similar to a Drosophila protein involved in early embryogenesis and the structural organization of indirect flight muscle. The gene is located within the Smith-Magenis syndrome region on chromosome 17.
Gene details: NCBI Gene ID: 2314; HGNC ID: HGNC:3750; UniProt: Q13045; RefSeq (MANE Select): NM_002018.4; Chromosome location: 17p11.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.