SYBR Green qPCR primers for detecting human FDX1 (ferredoxin 1, also called Hepatoredoxin) gene expression by real-time PCR.
Human FDX1 qPCR Primer Set – ferredoxin 1
Also known as: ADX, FDX, LOH11CR1D
About this gene: FDX1 encodes a small iron-sulfur protein that transfers electrons from NADPH through ferredoxin reductase to mitochondrial cytochrome P450, involved in steroid, vitamin D, and bile acid metabolism.
Gene details: NCBI Gene ID: 2230; HGNC ID: HGNC:3638; UniProt: P10109; RefSeq (MANE Select): NM_004109.5; Chromosome location: 11q22.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.